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Logo: Institut für Mehrphasenprozesse/Leibniz Universität Hannover
Logo Leibniz Universität Hannover
Logo: Institut für Mehrphasenprozesse/Leibniz Universität Hannover
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Prof. Dr. Ir. Willem F. Wolkers


"REBIRTH"-Professorship Biomedical Process Technology
Institut für Mehrphasenprozesse

Telefon:0511 762 19353/ -19350
E-Mail:wolkersimp.uni-hannover.de

Bild von Prof. Dr. Ir.  Willem F. Wolkers

Forschungsinteressen:

 

  • Biothermodynamische Grundlagen der Kryokonservierung von Zellen und Geweben
  • FTIR- und DSC-Untersuchungen zur Stabilität makromolekularer Strukturen am Beispiel des Hautersatzes Alloderm und Herzklappen 
  • Lyophilisation von Stammzellen, Thrombozyten und biologische Scaffolds
  • Einsatz von Liposomen zur Abgabe von Arzneimitteln und zur Zellstabilisierung

 

Werdegang:

 

  • Studium „Molecular Sciences“, Wageningen University, NL (1992)
  • Promotion (1998)  „The role of Macromolecular Stability in Desiccation Tolerance“, Wageningen University (1998)
  • Post-Doc am Center for Biostabilization, University of California, Davis, USA (1998-2001); Assistant Researcher/Group leader (2001-2004)
  • Research Assistant Professor am Department of Mechanical Engineering, University of Minnesota, Minneapolis, USA seit 2006
  • Leiter der REBIRTH-Professur „Biomedical Process Technology“ am Institut für Mehrphasenprozesse seit 2007, Berufung zum W3-Professor seit 2011

 

Publikationen "peer-reviewed": 

h index = 32 (obtained from http://www.scopus.com, self citations excluded) 

 

Buchbeiträge: 

 

  • Wolkers, W.F., Oldenhof, H. editors, (2015) Cryopreservation and Freeze-Drying Protocols, in book series: Methods in Molecular Biology – Vol 1275, Springer, New York, 528 p, ISBN 978-1-4939-2192-8.
  • Wolkers, W.F. (2009) Biomedical FTIR spectroscopy of lipids. In A. Barth, P.I. Haris, (eds.), Biological and Biomedical Infrared Spectroscopy. pp. 272-287. ISBN 978-1-60750-045-2
  • Crowe, J.H., Crowe, L.M., Tablin, F., Wolkers, W.F., Oliver, A.E., Tsvetkova, N.M. (2004) Stabilization of cells during freeze-drying: the trehalose myth. In B.J. Fuller, N. Lane, E.E. Benson (eds.), Life in the frozen state. pp. 581-601.
  • He, X., Wolkers, W.F., Crowe, J.H., Swanlund, D.J., Bischof, J.C. (2002) Investigation of in situ thermal denaturation of proteins in Dunning At-rat prostate cancer cells using FTIR and DSC. Proceedings of ASME IMECE meeting 1-5.
  • Golovina, E.A., Wolkers, W.F., Hoekstra, F.A. (1997) Behaviour of membranes and proteins during natural seed ageing. In R.H. Ellis (ed.), Basic and applied aspects of seed biology. pp. 787-796.
  • Hoekstra, F.A., Wolkers, W.F., Buitink, J. Golovina, E.A. (1997) Desiccation tolerance and long-term structural stability, In R.H. Ellis (ed.), Basic and applied aspects of seed biology. pp. 1-12.

 

Abstracts: 

 

  • Wolkers, W.F. (2016) Freeze-dried decellularized heart valves for heart valve replacement, Cryobiology, 73 (3) Page 403.
  • Zhang, M., Oldenhof, H., Sieme, H., Wolkers, W.F. (2016) Combining endocytic and freezing-induced trehalose uptake for cryopreservation of mammalian cells, Cryobiology, 73 (3), Page 412.
  • Oldenhof, H., Ertmer, F., Schütze, S., Wolkers, W.F., Sieme, H. (2015) Use of xanthine-xanthine oxidase for generating sub-lethal oxidative damage in stallion sperm and studies on osmotic tolerance and cryosurvival, Cryobiology, 71 (3), Page 547.
  • Wolkers, W.F., Hilfiker A. (2015) Dry preservation of decellularized heart valves, Cryobiology, 71 (3) Pages 551-552.
  • Zhang, M., Oldenhof, H., Sieme, H., Wolkers, W.F. (2015) Towards dry preservation of mammalian cells, Cryobiology, 71 (3), Page 554.
  • Wolkers, W.F. (2014) Dry preservation of decellularized heart valve tissues, Cryobiology, 69 (3), Page 507.
  • Wolkers, W.F. (2013) Freeze-dried cells and tissues, Cryobiology 67 (3), Page 422.
  • Oldenhof, H., Heutelbeck, A., Wolkers, W.F., Sieme, H. (2013) Temperature-dependent tolerance to hypotonic stress and cryosurvival of stallion sperm, Cryobiology, 67 (3), Pages 406.
  • Oldenhof, H., Gojowsky, M., Wolkers, W.F., Sieme, H. (2011) Osmotic properties and membrane phase behavior during cooling of stallion sperm, Cryobiology, 63 (3), Pages 310-311.
  • Akhoondi, M., Oldenhof, H., Wolkers, W.F. (2011) Water transport processes during cooling of mammalian cells, Cryobiology, 63 (3), Page 311.
  • Stoll, C., Holovati, J.L., Acker, J.P., Wolkers, W.F. (2011) Liposomes that deplete cholesterol from red blood cells can be used to increase trehalose uptake and cryosurvival, Cryobiology, 63 (3), Page 318.
  • Oldenhof, H., Friedel, K., Sieme, H., Glasmacher, B., Wolkers W.F. (2011) Membrane phase behavior and water transport during freezing of stallion sperm, Cryobiology, 62 (1), Page 88.
  • Stoll, C., Holovati, J., Glasmacher, B., Acker, J., Wolkers, W.F. (2010) Liposomes for biopreservation of human erythrocytes, Cryobiology, 61 (3), Page 394.
  • Wolkers, W.F., Stoll, C., Holovati, J.L., Acker, J.P., Glasmacher, B. (2009) Effect of liposomes on storage stability of red blood cell membranes studied by FTIR spectroscopy, Cryobiology, 59 (3) Page 399.
  • Wolkers, W.F. (2008) Effects of biopreservation on membranes and proteins, Cryobiology, 57 (3), Page 317.
  • Devireddy, R., Choi, J.H., Hagiwara, M., Wolkers, W.F., Roberts, K.P., Bischof, J.C. (2008) Cellular biophysics during freezing of rat and mouse sperm, Cryobiology, 57 (3), Page 322.
  • Wolkers, W.F., Oldenhof, H., Glasmacher, B. (2008) Lyotropic lipid phase transitions during drying under controlled relative humidity studied by ATR-FTIR spectroscopy, Cryobiology, 57 (3), Page 335.
  • Balasubramanian, S.K., Bartelt, K., Wolkers, W.F., Bischof, J.C. (2007) Freeze Injury in mammalian cells predicted by membrane phase behavior, Cryobiology, 55 (3), Page 336.
  • Hagiwara, M., Wolkers, W.F., Roberts, K., Bischof, J.C. (2007) Lipid hydration levels during freezing of murine sperm: an FTIR study, Cryobiology, 55 (3), Page 340.
  • Wolkers, W.F., Shah, N., Morrissey, M., Sun, W.Q., Bischof, J.C. (2007) Effect of gamma sterilization on proteins in AlloDermTM regenerative tissue matrix, Cryobiology, 55 (3), Pages 349-350.
  • Ragoonanan, V., Srikanth, S., Wolkers, W.F., Bond, D.R., Flickinger, M.C., Aksan, A. (2006) Quantification of the desiccation response of Geobacter sulfurreducens at the molecular level, Cryobiology 53 (3), Pages 396-397.
  • Oldenhof, H., Narten, K., Bigalk, J., et al. (2017) Preservation of sperm chromatin during dried storage, Reproduction in Domestic Animals, 52, Supplement S1, Pages: 38-38.
  • Sassi, P., Caponi, S., Ricci, M., et al. (2015) Lipid membrane phase transition: IR versus light scattering techniques, European Biophysics Journal with Biophysics Letters, 44, Supplement 1, Pages S198-S198.
  • Oldenhof, H., Ertmer, F., Schuetze, S., et al. (2014) Sub-lethal oxidative damage results in decreased osmotic tolerance in stallion sperm, Reproduction in Domestic Animals, 49, Special Issue SI, Supplement 1, Pages 34-34.
  • Oldenhof, H., Blaesse, A-K, Bollwein, H., et al. (2012) Fluorometric assessment of the intracellular pH and potassium concentration of stallion sperm, Reproduction in Domestic Animals, 47, Special Issue SI, Supplement 2, Pages: 39-39.
  • Bernemann, I.; Spindler, R.; Manuchehrabadi, N.; et al. (2011) Cryopreservation of engineered tissues: CT-visualizaton of diffusion in scaffolds, International Journal of artificial Organs 34 (8), Special Issue SI, Pages 684-684.
  • Oldenhof, H., Friedel, K., Gojowsky, M., et al., (2011) Membrane phase behavior of stallion sperm from 'good' and 'poor' freezers, Reproduction in Domestic Animals, 46, Special Issue SI, Supplement 1, Pages 32-32.
  • Oldenhof, H., Blaesse, A-K., Wolkers, W.F., et al. (2011) Osmotic properties of subpopulations of stallion sperm, Reproduction in Domestic Animals, 46, Special Issue SI, Supplement 1, Pages: 32-32.
  • Roepke, T., Oldenhof, H., Leiding, C., et al. (2011) Liposomes for cryopreservation of bull sperm, 46, Special Issue SI, Supplement 1, Pages: 36-37.
  • Wolkers, W.F., (2011) Membrane hydraulic permeability properties of sperm during freezing, Reproduction in Domestic Animals, 46, Special Issue SI, Supplement 1, Pages 46-46.
  • Chakradeo, T., Szentivanyi, A., Wolkers, W., et al. (2010) Sustained delivery of proteins from electrospun fibres, International Journal of Artificial Organs, 33 (7), Special Issue SI, Pages 427-427.
  • Akhoondi, M., Glasmacher, B., Wolkers, W.F. (2010) Modeling cellular biophysical response of 3T3 cells during freezing, International Journal of Artificial Organs, 33 (7), Special Issue SI, Page 454.
  • Acker, J. P., Stoll, C., Stadnick, H., et al. (2010) Application of liposomes to improve the hypothermic storage of red blood cells, Vox Sanguinis, 99, Supplement 1, Pages 67-68.
  • Diekmann, U., Wolkers, W.F., Spindler, R., et al. (2010) Effects of cryoconservation on gamma-irradiated mouse embryonic feeder cells, Cryoletters 31 (2), Pages 174-175.
  • Spindler, Ralf; Wolkers, Willem F.; Glasmacher, Birgit (2010) Interaction of dimethyl sulfoxide and ethylene glycol with cells during freezing, Cryoletters, 31 (2), Pages 178-178.
  • Stoll, C., Holovati, J., Acker, J., et al. (2010) Application of specific liposomes for preservation of red blood cells alter the cell membrane composition, Cryoletters, 31 (2), Pages 184-185.
  • Friedel, K., Oldenhof, H., Sieme, H., et al. (2010) Membrane phase behavior during freezing of stallion sperm, Cryoletters, 31 (2), Pages 188-189.
  • Wolkers, W.F., Diekmann, U., Mueller, T., et al. (2010) Cryomicroscopy and FTIR studies on gamma treated fibroblast feeder cells during freezing, Cryoletters, 31 (2), Pages 192-193.
  • Bernemann, I., Spindler, R., Manuchehrabadi, N., et al. (2010) Dimethyl sulfoxide diffusion in tissue-engineered collagen scaffolds visualized by computer tomography, Cryoletters, 31 (2), Pages 193-194.
  • Oldenhof, H., Friedel, K., Wolkers, W.F., et al. (2010) Membrane permeability parameters during freezing of stallion sperm, determined by Fourier transform infrared spectroscopy, Reproduction in Domestic Animals 45, Supplement 1, Pages 37-37.
  • Schmid, S., Henning, H., Oldenhof, H., et al. (2010) Effect of hypothermic liquid storage on the lipid architecture and behaviour of boar sperm plasma membranes, Reproduction in Domestic Animals 45, Supplement 1, Page 48.
  • Diekmann, U., Wolkers, W.F., Spindler, R., et al. (2009) Design of a "bench top" quality control panel for mouse embryonic feeder cells, Human Gene Therapy, 20 (11), Pages 1430-1430.
  • Wolkers, W.F., Diekmann, U., Mueller, T., et al. (2009) Cryomicroscopy and FTIR studies on mouse embryonic fibroblast feeder cells during cryopreservation, Human Gene Therapy, 20 (11), Pages: 1434-1434.
  • Spindler, R., Wolkers, W.F., Glasmacher, B., (2009) Effect of Me2SO on membrane phase behavior and protein denaturation of human pulmonary endothelial cells studied by in situ FTIR spectroscopy, Cryoletters, 30 (2), Pages 164-164.
  • Venkatasubramanian, R., Wolkers, W.F., Soule, C., et al. (2009) Effect of tissue dehydration on smooth muscle cell contractility, collagen matrix structure and overall artery biomechanics, Proceedings of the ASME summer bioengineering conference 2008, Pts. A and B, Pages 261-262.
  • Wolkers, W.F., Shah, N.B., Morrissey, M., et al. Biological skin replacement: the need for sterilization, International Journal of Artificial Organs, 31 (7), Pages 583-583.
  • Zhang, L., Wolkers, W.F., Aksan, A. (2007) Thermal damage distribution in human cornea following thermal Keratoplasty: An FTIR study, Proceeding of the ASME summer bioengineering conference - 2007, Pages 79-80.
  • Balasubramanian, S.K., Wolkers, W.F., Bischof, J.C. (2007) Thermal "fingerprinting" of cells using FTIR, Proceeding of the ASME summer bioengineering conference - 2007, Pages 87-88.
  • Tablin, F., Wolkers, W.F., Tsvetkova, N.M., et al. (2005) Trehalose maintains membrane lipid domain organization in freeze-dried rehydrated platelets: Implications for long-term storage in the dry state. Abstracts of papers of the American chemical society 229: U641-U641 073-COLL Part 1.
  • Auh, J.H., Wolkers, W.F., Tablin, F., et al. Calcium mobilization in freeze dried human platelets. Biophysical Journal 86 (1): 576A-577A Part 2 Suppl. S 2004.
  • Ricker, J.V., Tsvetkova, N.M., Wolkers W.F. et al. Phase behavior of air-dried binary lipid mixtures. Biophysical Journal 84 (2): 47A-48A Part 2 Suppl. S 2003.
  • Wolkers, W.F., Looper, S.A., Fontanilla, R.A., et al. Temperature dependence of fluid phase endocytosis coincides with membrane properties of pig platelets. Biophysical Journal 84 (2): 210A-210A Part 2 Suppl. S 2003.
  • Tsvetkova, N., Horvath. I., Torok, Z., et al. Interactions of small heat-shock proteins with membranes. Biophysical Journal 82 (1): 144A-145A 707 Part 2, 2002.
  • Ricker, J.V., Wolkers, W.F., Tsvetkova, N.M. et al. Trehalose maintains lipid phase separation in model and biological membranes. Molecular Biology of the Cell 13: 2032 2002.
  • Tablin, F., Looper, S.A., Wolkers, W.F., et al. Trehalose loaded freeze-dried mouse platelets circulate in vivo: Evidence for a novel platelet product. Blood 100 (11): 2787 Part 1 2002.
  • Tablin, F., Ricker, J.V., Tsvetkova, N.M., et al. Trehalose stabilizes membrane microdomains in freeze-dried platelets. Blood 100 (11): 2788 Part 1 2002.
  • Wolkers, W.F., Crowe, L.M., Hoekstra, F.A., et al. A FTIR spectroscopy study of carbohydrate glasses. Biophysical Journal 82 (1): 179 Part 2 2002.
  • Leidy, C., Wolkers, W.F., Oliver, A.E. et al. Using FTIR to measure the partitioning of cholesterol in sphingomyelin/DPPC mixtures: Keys for the structural factors that determine the formation of cholesterol-enriched domains. Biophysical Journal 82 (1): 753 Part 2 2002.
  • Tablin, F., Wolkers, W.F., Walker, N.J., et al. Trehalose loaded, freeze-dried human platelets are functional and retain normal protein structure and membrane phospholipid phase transitions. Blood 96 (11): 261 Part 1 2000.
  • Crowe, L.M., Wolkers, W.F., Crowe, J.H. The "glass" transition in a model system. Biophysical Journal 78 (1): 2870Pos Part 2 2000.
  • Wolkers, W.F., Hoekstra, F.A. Protection of biomolecules in dehydrating pollen: A FT-IR microspectroscopy study. Progress in Biophysics & Molecular Biology 65: PA429-PA429 Suppl. 1 1996.
  • Wolkers, W.F., Gregory, J.D., Churchich J.E., et al. Kinetic fluorescence and magnetic resonance studies of brain pyridoxal kinase. Faseb Journal 5 (5): A1149-A1149 Part 2 1991.

 

Vorträge

 

  • Wolkers W.F. (2017) Annual Meeting of the Society for Cryobiology, Hefei, China, Dry preservation of decellularized cardiovascular scaffolds.
  • Zhang M., Oldenhof H., Sydykov B., Bigalk J., Sieme H., Wolkers W.F. (2017) Annual Meeting of the Society for Cryobiology, Hefei, China, Use of trehalose to preserve DNA integrity in freeze-dried mammalian cells.
  • Oldenhof H., Miao Zhang M., Narten K., Bigalk J., Sydykov B., Wolkers W.F., Sieme H. (2017) Annual Meeting of the Society for Cryobiology, Hefei, China, Preservation of chromatin in freeze-dried sperm stored under accelerated aging conditions.
  • Vásquez-Rivera A., Oldenhof H., Haverich A., Korossis S., Hilfiker A., Wolkers W.F. (2017) Annual Meeting of the European Society for Artificial Organs (ESAO), Vienna, Austria, Effect of porosity in freeze-dried decellularized porcine pulmonary heart valves on proteins and biomechanical properties.
  • Vásquez-Rivera A., Oldenhof H., Haverich A., Korossis S., Hilfiker A., Wolkers W.F. (2017) Annual Meeting of the German Society of Biomechanics (DGfB), Freeze-dried heart valves.
  • Zouhair S., Aguiari P., Iop L., Korossis S., Wolkers W.F., Gerosa G. (2017) Annual Meeting of the German Society of Biomechanics (DGfB), Advanced preservation methodologies of for decellularized cardiovascular scaffolds.
  • Wolkers W.F. (2016) Annual Meeting of the Society for Cryobiology, Ottawa, Ontario, Canada, Freeze-dried decellularized heart valves for heart valve replacement.
  • Wolkers, W.F., Hilfiker, A. (2015) Annual Meeting of the Society for Cryobiology, Ostrava, Czech Republic, Dry preservation of decellularized heart valves.
  • Wolkers, W.F. (2014) Annual Meeting of the Society for Cryobiology, Savannah, GA, USA, Dry preservation of decellularized heart valve tissues.
  • Wolkers, W.F. (2013) Annual Meeting of the Society for Cryobiology, Washington DC, USA, Freeze-dried cells and tissues.
  • Oldenhof, H., Heutelbeck, A., Wolkers, W.F., Sieme, H. (2013) Annual Meeting of the Society for Cryobiology, Washington DC, USA, Temperature-dependent tolerance to hypotonic stress and cryosurvival of stallion sperm.
  • Wang S., Oldenhof, H., Hilfiker, A., Harder M., Kabelac, S., Wolkers, W.F. (2013) Thermodynamik Kolloquium, Hamburg, Germany, Diffusion of protective agents in heart valve scaffolds (Presentation).
  • Wolkers, W.F. (2013) Annual Meeting of the Society for Low Temperature Biology, Hannover, Germany, Freeze-dried cells and tissues.
  • Wolkers, W.F. (2012) Frontiers in water biophysics, Perugia, Italy, Water transport processes during freezing of cells.
  • Wang S., Oldenhof, H., Hilfiker, A., Harder M., Wolkers, W.F. (2012) Thermodynamik Kolloquium, Hamburg, Germany, Thermal fingerprinting and solvent accessibility of proteins in heart valve scaffolds.
  • Akhoondi, M., Wolkers, W.F. (2011) Thermodynamik Kolloquium, Hamburg, Germany, Water transport processes during freezing of mammalian cells.
  • Akhoondi, M., Oldenhof, H., Wolkers W.F. (2011) Annual Meeting of the Society for Cryobiology, Washington DC, USA, Water transport processes during cooling of mammalian cells.
  • Stoll, C., Holovati, J.L., Acker, J.P., Wolkers W.F. (2011) Annual Meeting of the Society for Cryobiology, Washington DC, USA, Liposomes that deplete cholesterol from red blood cells can be used to increase trehalose uptake and cryosurvival.
  • Oldenhof, H., Gojowsky, M., Wolkers, W.F., Sieme H. (2011) Annual Meeting of the Society for Cryobiology, Washington DC, USA, Osmotic properties and membrane phase behavior during cooling of stallion sperm.
  • Tablin, F., Wolkers, W.F., Tsvetkova, N.M., et al. (2005) Trehalose maintains membrane lipid domain organization in freeze-dried rehydrated platelets: Implications for long-term storage in the dry state. Abstracts of papers of the American chemical society 229: U641-U641 073-COLL Part 1.
  • Tablin, F., Looper, S.A., Wolkers, W.F., et al. Trehalose loaded freeze-dried mouse platelets circulate in vivo: Evidence for a novel platelet product. Blood 100 (11): 2787 Part 1 2002.
  • Tablin, F., Ricker, J.V., Tsvetkova, N.M., et al. Trehalose stabilizes membrane microdomains in freeze-dried platelets. Blood 100 (11): 2788 Part 1 2002.
  • Leidy, C., Wolkers, W.F., Oliver, A.E. et al. Using FTIR to measure the partitioning of cholesterol in sphingomyelin/DPPC mixtures: Keys for the structural factors that determine the formation of cholesterol-enriched domains. Biophysical Journal 82 (1): 753 Part 2 2002.
  • Tablin, F., Wolkers, W.F., Walker, N.J., et al. Trehalose loaded, freeze-dried human platelets are functional and retain normal protein structure and membrane phospholipid phase transitions. Blood 96 (11): 261 Part 1 2000.

 

Posterpresentationen

 

  • Sydykov B., Oldenhof H., Oliveira Barros, L., Sieme H., Wolkers W.F. (2017) Annual Meeting of the European Biophysical Societies' Association (EBSA), Edinburgh, Schotland, Membrane permeabilization of egg phosphatidylcholine liposomes induced by cryoprotective agents.
  • Wang S., Oldenhof, H., Dai, X., Haverich, A., Hilfiker, A., Harder M., Wolkers, W.F. (2013) Annual Meeting of the Society for Low Temperature Biology, Hannover, Germany, Protein stability and solvent accessibility in decellularized pulmonary heart valve tissues.
  • Oldenhof, H., Akhoondi, M., Teuntchou Noumeni, C., Sieme, H., Wolkers, W.F. (2013) Annual Meeting of the Society for Low Temperature Biology, Hannover, Germany, Membrane phase changes during cooling and drying of mammalian cells.
  • Akhoondi, M., Oldenhof, H., Sieme H. Wolkers, W.F. (2012) Frontiers in water biophysics, Perugia, Italy, Water transport processes during freezing of cells in the presence of cryoprotective agents.
  • Wang, S., Goecke, T., Meixner, C., Haverich, A., Hilfiker, A., Wolkers, W.F. (2012) Frontiers in water biophysics, Perugia, Italy, Freeze-dried heart valve matrix implants.
  • Oldenhof, H., Gojowsky M., Wang, S., Wolkers, W.F., Sieme, H. (2012) Frontiers in water biophysics, Perugia, Italy, Mode of action of different cryoprotective agents used for cryopreservation of stallion sperm (Poster).
  • Bernemann I., Spindler R., Manuchehrabadi N., Choi J.H., Wolkers W.F., Bischof J.C., Glasmacher B. (2011) Congress of the European Society for Artificial Organs (ESAO), Porto, Portugal, Cryopreservation of engineered tissues: CT-Visualization of CPA diffusion in scaffolds.
  • Wolkers, W., Glasmacher, B. (2008) International Symposium on Biothermodynamics, Frankfurt am Main, Germany, Protein stability in thrombocytes and erythrocytes studied in situ by Fourier transform infrared spectroscopy.
  • Wolkers, W., Oldenhof, H., Glasmacher, B. (2008) Annual Meeting of the Society for Cryobiology, Charlotte, USA, Lyotropic lipid phase transitions during drying under controlled relative humidity studied by ATR-FTIR spectroscopy.
  • Szentivanyi, A., Wolkers, W., Glasmacher, B. Bio § Biopolymers Biannual meeting of the GDCh- division of Macromolecular Chemistry (2008), Processing of water-based protein/polymer solutions by electrospinning.
  • Wolkers, W., Glasmacher, B. (2008) International Symposium on Biothermodynamics, Frankfurt am Main, Germany, Protein stability in thrombocytes and erythrocytes studied in situ by Fourier transform infrared spectroscopy.
  • Spindler, R., Wolkers, W., Glasmacher, B. Society for Low Temperature Biology (2008), Copenhagen, Denmark. Effect of Me2SO on membrane phase behavior and protein denaturation of human pulmonary endothelial cells studied by in situ FTIR spectroscopy.
  • Wolkers, W., Oldenhof, H., Glasmacher, B. (2008) Annual Meeting of the Society for Cryobiology (2008), Charlotte, USA, Lyotropic lipid phase transitions during drying under controlled relative humidity studied by ATR-FTIR spectroscopy.
  • Szentivanyi, A., Wolkers, W., Glasmacher, B. (2008) Bio § Biopolymers Biannual meeting of the GDCh- division of Macromolecular Chemistry, Processing of water-based protein/polymer solutions by electrospinning .
  • Spindler, R., Wolkers, W., Glasmacher, B. (2008) Society for Low Temperature Biology, Copenhagen, Denmark. Effect of Me2SO on membrane phase behavior and protein denaturation of human pulmonary endothelial cells studied by in situ FTIR spectroscopy. .
  • Auh, J.H., Wolkers, W.F., Tablin, F., et al. Calcium mobilization in freeze dried human platelets. Biophysical Journal 86 (1): 576A-577A Part 2 Suppl. S 2004.
  • Ricker, J.V., Tsvetkova, N.M., Wolkers W.F. et al. Phase behavior of air-dried binary lipid mixtures. Biophysical Journal 84 (2): 47A-48A Part 2 Suppl. S 2003.
  • Wolkers, W.F., Looper, S.A., Fontanilla, R.A., et al. Temperature dependence of fluid phase endocytosis coincides with membrane properties of pig platelets. Biophysical Journal 84 (2): 210A-210A Part 2 Suppl. S 2003.
  • Tsvetkova, N., Horvath. I., Torok, Z., et al. Interactions of small heat-shock proteins with membranes. Biophysical Journal 82 (1): 144A-145A 707 Part 2, 2002.
  • Ricker, J.V., Wolkers, W.F., Tsvetkova, N.M. et al. Trehalose maintains lipid phase separation in model and biological membranes. Molecular Biology of the Cell 13: 2032 2002.
  • Wolkers, W.F., Crowe, L.M., Hoekstra, F.A., et al. A FTIR spectroscopy study of carbohydrate glasses. Biophysical Journal 82 (1): 179 Part 2 2002.
  • Crowe, L.M., Wolkers, W.F., Crowe, J.H. The "glass" transition in a model system. Biophysical Journal 78 (1): 2870Pos Part 2 2000.
  • Wolkers, W.F., Hoekstra, F.A. Protection of biomolecules in dehydrating pollen: A FT-IR microspectroscopy study. Progress in Biophysics & Molecular Biology 65: PA429-PA429 Suppl. 1 1996.
  • Wolkers, W.F., Gregory, J.D., Churchich J.E., et al. Kinetic fluorescence and magnetic resonance studies of brain pyridoxal kinase. Faseb Journal 5 (5): A1149-A1149 Part 2 1991.

 

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