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Logo: Institute for Multiphase Processes
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Logo: Institute for Multiphase Processes
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Oleksandr Gryshkov, PhD


Speaker of the Research Group Low Temperature Applications / Cryotechnology (IMP) and RG Glasmacher (NIFE)
Institut for Multiphase Processes
Room 231
Callinstrasse 36
30167 Hannover

Laboratory facility 1
Nienburgerstrasse 1
30167 Hannover

Laboratory facility 2
Lower Saxony Center for Biomedical engineering, Implant research and Development
AG Glasmacher, Room 2020
Stadtfelddamm 34, 30625 Hannover

phone:+49(0) 511 762 3822 / 0511 532 1342
fax:+49(0) 511 762 3031
mail:gryshkovimp.uni-hannover.de

Bild von  Oleksandr Gryshkov, PhD

Project management

Wege in die Forschung II: "ArtiBone: Cryopreservation of tissue-engineered bone substitutes" (01.09.2016-31.12.2018)

Current Research Topics

  • High voltage encapsulation of stem cells in alginate
  • Cryopreservation of TE scaffolds and native tissues
  • Engineering of bioactive coatings onto bio-SiC, magnesium and Ti-based medical implants
  • Cryopreservation of adherent MSCs

Curriculum Vitae

since 04/2015 - Post Doc, Institute for Multiphase Processes (IMP), Gottfried Wilhelm Leibniz University Hannover, Germany

01/2015 - PhD in Biomedical Engineering, Medical School Hannover and IMP

10/2011 - 03/2015 - PhD Candidate, IMP

11/2012 - 10/2013 - Distance learning (Marketing), Kharkiv State University for Trade, Kharkiv, Ukraine

11/2009 - 10/2012 - PhD Candidate, Institute of Semiconductor Physics, National Academy of Sciences of Ukraine, Kyiv, Ukraine

09/2007 - 06/2009 - Master of Sciences (Sold-state physics, teacher of physics), National Taras Shevchenko Univeristy, Kiew, Ukraine

09/2003 - 06/2007 - Baschelor of Sciences (Physics), National Taras Shevchenko Univeristy, Kiew, Ukraine

Publications

Articles

2019

  • O. Gryshkov, M. Müller, S. Leal-Marin, V. Mutsenko, S. Suresh, V.M. Kapralova, B. Glasmacher. Advances in the application of electrohydrodynamic fabrication for tissue engineering. J. Phys. Conf. Ser. 2019;1236 012024, doi 10.1088/1742-6596/1236/1/012024.
  • A. Tretikaov, V. Kapralova, N. Sudar, O. Gryshkov, B. Glasmacher. Dielectric properties of PVDF-based thin films and electrospun mats. J. Phys. Conf. Ser. 2019;1236 012009, doi 10.1088/1742-6596/1236/1/012009.
  • V. Mutsenko, O. Gryshkov, O. Rogulska, A. Lode, A.Yu. Petrenko, M. Gelinsky, B. Glasmacher, H. Ehrlich. Chitinous Scaffolds from Marine Sponges for Tissue Engineering. In: A.H. Choi, B. Ben-Nissan (eds.) (2018). Marine-Derived Biomaterials for Tissue Engineering Applications, Springer Series in Biomaterials Science and Engineering (SSBSE) 2019: 285-307, doi 10.1007/978-981-13-8855-2_13.
  • M. Tymkovych, O. Avrunin, O. Gryshkov, V. Semenets, B. Glasmacher. Ice Crystals Microscopic Images Segmentation Based on Active Contours. IEEE 39th International Conference on Electronics and Nanotechnology (ELNANO), Kyiv, Ukraine, 2019, pp. 493-496, doi: 10.1109/ELNANO.2019.8783332.

2018

  • S. Suresh, O. Gryshkov, B. Glasmacher. Impact of setup orientation on blend electrospinning of Poly-ε-caprolactone-Gelatin scaffolds for vascular tissue engineering. Int J Artif Organ 2018;41(11): 801-810.
  • F. Al Halabi, O. Gryshkov, A. I. Kuhn, V. M. Kapralova, B. Glasmacher. Force induced piezoelectric effect of PVDF and PVDF-TrFE scaffolds. Int J Artif Organ 2018;41(11): 811-822.
  • M.V. Prykhodko, M.Y. Tymkovych, O.G. Avrunin, V.V. Mutsenko, O. Gryshkov, B. Glasmacher. Image processing for automated microscopic analysis of ice recrystallization process during isothermal annealing. Int J Bioelectromagnetism 2018;20(1): 72-75.
  • Ya.V. Nosova, O.G. Avrunin, N.O. Shusliapina, O. Gryshkov, B. Glasmacher. The distribution of the velocity and pressure fields in the nasal cavity at different respiration modes. Int J Bioelectromagnetism 2018;20(1): 68-70.
  • V.M. Kapralova, N.L. Vaskova, E.B. Shadrin, A.V. Ilinsky, O. Gryshkov, B. Glasmacher. Cerebrospinal fluid thermoimpedancemetry as a method of brain diseases diagnostics. Int J Bioelectromagnetism 2018;20(1): 63-65.
  • I.S. Bondarenko, O.G. Avrunin, O. Gryshkov, B. Glasmacher. Possibilities of joint application of acoustic radiation and direct magnetic field for biomedical research. Int J Bioelectromagnetism 2018;20(1): 66-67.
  • F. Al Halabi, O. Gryshkov, A. I. Kuhn, V. M. Kapralova, B. Glasmacher. Piezoelectric properties of PVDF and PVDF-TrFE electrospun materials for nerve regeneration. J Biomed Radioelectron 2018: 123-126.

2017

  • M.Yu. Tymkovych, O.G. Avrunin, V.G. Paliy, M. Filzow, O. Gryshkov et al. Automated method for structural segmentation of nasal airways based on cone beam assisted computed tomography. Photonics Applications in Astronomy, Communications, Industry, and High-Energy Physics Experiments 2017, Edition: Proceedings of SPIE, Chapter: 10445, Editors: Romaniuk Ryszard S., Linczuk Maciej, pp.104453F
  • V. V. Mutsenko, O. Gryshkov, L. Lauterboeck, et al. Novel chitin scaffolds derived from marine sponge Ianthella basta for tissue engineering approaches based on human mesenchymal stromal cells:Biocompatibility and cryopreservation. Int J Biol Macromol 2017;104(B): 1955-1965
  • A. Chatterjee, D. Saha, H. Niemann, O. Gryshkov, B. Glasmacher, N. Hofmann. Effects of cryopreservation on the epigenetic profile of cells. Cryobiology 2017;74: 1-7

http://www.sciencedirect.com/science/article/pii/S0011224016304643

2016

  • O. Gryshkov, N.I. Klyui, V.P. Temchenko, et al. Porous biomorphic silicon carbide ceramics coated with hydroxyapatite as prospective materials for bone implants. Mater Sci Eng C 2016;68: 143-152
  • L. Lauterboeck, O. Gryshkov, N. Hofmann, et al. Importance of controlled ice formation for efficient biobanking. Refrigeration Science and Technology 2016;F126957: 84-90. doi: 10.18462/iir.cals.2016.0007

2015

  • A. Repanas, W.F. Wolkers, O. Gryshkov, M. Müller, B. Glasmacher. PCL/PEG electrospun fibers as drug carriers for the controlled delivery of dipyridamole. Journal of In Silico & In Vitro Pharmacology 2015;1(1:2) in press
  • A. Repanas, W.F. Wolkers, O. Gryshkov, et al. Coaxial electrospinning as a process to engineer biodegradable polymeric scaffolds as drug delivery systems for anti-inflammatory and anti-thrombotic pharmaceutical agents. Clinical & Experimental Pharmacology 2015;5: 192, DOI:10.4172/2161-1459.1000192
  • O. Gryshkov, N. Hofmann, L. Lauterboeck, T. Mueller, D. Pogozhykh, B. Glasmacher. Multipotent Stromal Cells Derived from Common Marmoset Callithrix Jacchus within Alginate 3D Environment: Effect of Cryopreservation Procedures. Cryobiology 2015:71(1): 103-111

 2014

  • O. Gryshkov,  D. Pogozhykh, N. Hofmann, O. Pogozhykh, T. Mueller, B. Glasmacher. Encapsulating Non-Human Primate Multipotent Stromal Cells in Alginate via High Voltage for Cell-Based Therapies and Cryopreservation. PLoS One 2014;9(9): e107911
  • O. Gryshkov, D. Pogozhykh, N. Hofmann, H. Zernetsch, B. Glasmacher. Process engineering of high voltage alginate encapsulation of mesenchymal stem cells. Mater Sci Eng C 2014;36: 77-83

2013

  • M.I. Klyui, V.P. Temchenko, O.P. Gryshkovet al. Bio-SiC ceramics coated with hydroxyapatite using gas-detonation deposition: An alternative to titanium-based medical implants. Functional materials 2013;20(2): 163-171

2012

  • M.I. Klyui, V.P. Temchenko, A.V. Makarov, O.P. Gryshkov, V.O. Yukhymchuk. Properties of micro- and nano-structured silicon layers produced by gas-detonation deposition. Technical Electrodynamics 2012, Thematic Issue: 198-203

2011

  • N.I. Klyui, V.P. Temchenko, A.P. Gryshkovet al. Properties of the hydroxyapatite coatings obtained by gas-detonation deposition onto titanium substrates. Functional Materials 2011;18(3): 285-292

Abstracts

2019

  • O. Gryshkov, V. Mutsenko, S. Suresh, B. Glasmacher. Cryopreservation of cell-seeded electrospun materials: towards biobanking of tissue-engineered constructs. Int J Artif Organs 2019;42(8): 399.
  • E. Anastassopoulos, V. Mutsenko, O. Gryshkov, B. Glasmacher. Development of efficient cryopreservation protocols based on biomimetics.  Int J Artif Organs 2019;42(8): 457.
  • V. Mutsenko, M. Chasnitsky, V. Sirotinskaya, M. Müller, B. Glasmacher, I. Braslavsky, O. Gryshkov. Directional and conventional freezing of cell-seeded electrospun fiber mats.  Int J Artif Organs 2019;42(8): 438.
  • V. Mutsenko, D. Tarusin, J. Dermol-Černe, B. Glasmacher, D. Miklavčič, M. Gelinsky, O. Gryshkov. Strategies towards efficient cryopreservation of cell-free and cell-seeded scaffolds.  Int J Artif Organs 2019;42(8): 469.
  • A.I. Kuhn, O. Gryshkov, K.-V. Peinemann, B. Glasmacher. Polarization of piezoelectric polyvinylidene fluoride via electrospinning for active filter membranes.  Int J Artif Organs 2019;42(8): 410.
  • O. Pogozhykh, N. Hofmann, D. Pogozhykh, M. Müller, S. Aufderbeck, B. Glasmacher, O. Gryshkov, R. Blasczyk, M. Börgel, C. Figueiredo. Cryopreservation does not affect the regenerative potential of human amniotic membrane.  Int J Artif Organs 2019;42(8): 398.
  • S. Leal-Marin, O. Gryshkov, O. Pogozhykh, N. Hofmann, B. Glasmacher. Towards biobanking of tissue-engineered amniotic membrane.  Int J Artif Organs 2019;42(8): 428.
  • S. Suresh, S. M. Kidwai, O. Gryshkov, B. Glasmacher. Multipotent stromal cell growth assessment on polycaprolactone-gelatin hybrid electrospun scaffolds.  Int J Artif Organs 2019;42(8): 402.
  • A. Tretiakov, V. M. Kapralova, N. T. Sudar, O. Gryshkov, B. Glasmacher. A dielectric study of piezoelectric fibrous mats with electrical switching effect for neural tissue implants.  Int J Artif Organs 2019;42(8): 409.
  • M. Tymkovych, O. Avrunin, O. Gryshkov, K. Selivanova, V. Mutsenko, B. Glasmacher. Multiscale quantitative analysis of microscopic images of ice crystals.  Int J Artif Organs 2019;42(8): 429.
  • O. Avrunin, I. Bondarenko, O. Gryshkov, B. Glasmacher, S. Bondarenko, O. Krevsun, M. Rakhimova. Acoustomagnetic detection of magnetic nanoparticles in a model sample of the biologycal substance.  Int J Artif Organs 2019;42(8): 429.
  • O. Avrunin, Y. Nosova, O. Gryshkov, B. Glasmacher, N. Shushliapina. Research of laminar boundary layer influence of the air flow on the mucous membrane of the nasal cavity.  Int J Artif Organs 2019;42(8): 430.

2018

  • O. Gryshkov, M. Tymkovych, O. Avrunin, B. Glasmacher. Image-series analysis for recrystallization process evaluation. In Proceedings of the 1st International Scientific and Practical Conference “Information Systems and Technologies in Medicine”, Kharkiv, Ukraine, 28-30.11.2018, p. 256-258.
  • O. Gryshkov, V. Mutsenko, M. Tymkovych, D. Tarusin, V. Sirotinskaya, I. Braslavsky, O. Avrunin, B. Glasmacher. Advances in cryopreservation of alginate-encapsulated stem cells and analysis of cryopreservation outcome. Cryobiology 2018:85; 156.
  • O. Gryshkov, M. Tymkovych, O. Avrunin, B. Glasmacher. Optimal cryopreservation yields intact alginate micro-spheres and high cell viability after thawing. Cryobiology 2018:85; 178.
  • V. Mutsenko, A. Barlič, T. Pezić, J. Dermol-Černe, B. Sydykov, O. Gryshkov, W.F. Wolkers, D. Miklavčič, B. Glasmacher. Me2SO- and serum-free cryopreservation of mesenchymal stromal cells using electroporation of sugars. Cryobiology 2018:85; 156-157.
  • V. Mutsenko, O. Gryshkov, B. Sydykov, A. Beck, D. Dipresa, A. Lode, T. El Khassawna, A. Petrenko, S. Korossis, W.F Wolkers, M. Gelinsky, B. Glasmacher. ‘In air’ cryopreservation of mesenchymal stromal cells on 3D collagen-hydroxyapatite-scaffolds. Cryobiology 2018:85; 145.
  • V. Mutsenko, D. Tarusin, B. Sydykov, D. Zaragotas, A. Simaioforidou, C. Rozanski, O. Gryshkov, W.F. Wolkers, I. Braslavsky, E. Anastassopoulos, B. Glasmacher. Study of natural cryoprotective agents for cryopreservation of 3D tissue-engineered scaffolds. Cryobiology 2018:85; 176.
  • S. Suresh, MS. Balamuruganandam, O. Gryshkov, B. Glasmacher. Impact of heterogeneous microstructure of PCL/gelatin electrospun scaffolds on the infiltration of fibroblasts. Int J Artif Organ 2018:41(9); 501.
  • C. Esch, M. Müller, O. Gryshkov, S. Knigge, B. Glasmacher. Visualization Of Hemodynamics: Synthesis Of Artificial Erythrocytes With Encapsulated Piv-Particles Via Microfluidic Systems And Electro-Spraying. Int J Artif Organ 2018:41(9); 568.
  • A.I. Kuhn, F. Al Halabi, O. Gryshkov, B. Glasmacher. Modification of electrospun fibrous polyvinylidene fluoride mats with polyaniline to improve piezoelectric conductivity. Int J Artif Organ 2018:41(9); 547.
  • B. Glasmacher, M. Bode, O. Gryshkov, M. Mueller. An Engineer’S Contribution To Efficient Vascular Replacement Grafts. Int J Artif Organ 2018:41(9); 529.

2017

  •  S. Suresh, O. Gryshkov, B. Glasmacher. Controlled infiltration of cells into electrospun scaffolds fabricated for applications in small diameter vascular grafts. Biomed. Eng.-Biomed. Tech. 2017; 62(s1): S461.
  • S. Suresh, M. Balamuruganandam, O. Gryshkov, B. Glasmacher. Controlled infiltration of cells into electrospun scaffolds fabricated for application in small diameter vascular grafts. Int J Artif Organs 2017; 40(8): 466.
  • F. AL Halabi, O. Gryshkov, M. Marc, V. Kapralova, B. Glasmacher. Force induced piezoelectric effect of PVDF and PVDF-TRFE scaffolds. Int J Artif Organs 2017; 40(8): 467.
  • V. Mutsenko, O. Gryshkov, B. Glasmacher. Cryopreservation of amnion-derived multipotent stem cells within alginate- and collagen-based polymeric matrices. CryoLetters 2018:39(1); 85.

2016

  • V.V. Mutsenko, O. Gryshkov, S. Knaack, et al. Cryopreservation of biomimetic bone matrix with non-human primate mesenchymal stromal cells. Cryobiology 2016;73(3): 433.
  • V.V. Mutsenko, O.P. Gryshkov, Yu.A. Petrenko, et al. The impact of sucrose pretreatment on survival of mesenchymal stromal cells, cryopreserved in suspension and adherent state. Cryobiology 2016;73(3): 402.
  • S. Suresh, O. Gryshkov, B. Glasmacher. Controlled infiltration of cells into spherical polymeric scaffolds. Int J Artif Organs 2016; 39(7): 373.
  • B. Glasmacher, L. Lauterboeck, D. Saha, A. Chatterjee, S. Suresh, T. Rittinghaus, O. Gryshkov. Cell and tissue encapsulation in alginate for safe cryopreservation. Cryobiology 2016;73(3): 407.
  • O. Gryshkov, N. Klyui, V. Temchenko, B. Glasmacher. Deposition of bioactive and bioinert ceramic coatings on magnesium using detonation. Biomed Tech 2016;61: 198.
  • S. Suresh, O. Gryshkov, B. Glasmacher. Development of Volvox spheres for the co-culture of cardiovascular cells for tissue engineering. Biomed Tech 2016;61: 89.
  • F. AL Halabi, A. Repanas, M. Müller, O. Gryshkov, B. Glasmacher. Piezoelectric effect of PFDV and PVDF-TrFE scaffolds. Biomed Tech 2016;61: 89.

2015

  • O. Gryshkov, N. Klyui, V. Temchenko, et al. Porous bio-SiC ceramics from wood: approaching new medical implants. World Congress on Medical Physics and Biomedical Engineering – IUPESM 2015, 7-12 June 2015, Toronto, Canada, pp. 283-284.
  • O. Gryshkov, H. Zernetsch, N. Hofmann, B. Glasmacher. Alginate encapsulation: a solution for controlled infiltration of cells within artificial fiber constructs. World Congress on Medical Physics and Biomedical Engineering – IUPESM 2015, 7-12 June 2015, Toronto, Canada, pp. 205.
  • O. Gryshkov, L. Lauterboeck, N. Hofmann, B. Glasmacher. Temperature of ice formation affects integrity of alginate 3D constructs after cryopreservation. World Congress on Medical Physics and Biomedical Engineering – IUPESM 2015, 7-12 June 2015, Toronto, Canada, pp. 535.
  • V.M. Kapralova, D.D. Karov, V.V. Loboda, O. Gryshkov, B. Glasmacher. Study of mechanical and degradation behavior of new copolymers for medical implants. 11th German-Russian Conference on Biomedical Engineering, 17-19 June 2015, Aachen, Germany, pp. 103-105.
  • O. Gryshkov, N. Klyui, V. Temchenko, V. Dubok, V. Kyselyov, A. Belyaev, B. Glasmacher. Deposition of bioactive coatings on porous bio-SiC ceramics derived from wood. 11th German-Russian Conference on Biomedical Engineering, 17-19 June 2015, Aachen, Germany, pp. 107-109.
  • M. Tymkovych, O. Gryshkov, O. Avrunin, A. Kern, B. Glasmacher. An approach to visualize alginate 3D structures with encapsulated cells for cell-based therapies and cryopreservation. 11th German-Russian Conference on Biomedical Engineering, 17-19 June 2015, Aachen, Germany, pp. 13-15.
  • O. Gryshkov, M. Tymkovych, O. Avrunin, et al. Visualization of alginate beads with encapsulated cells for cryopreservation and cell-based therapies. International Journal of Artificial Organs 2015;38(7): 408.

2013

  • O. Gryshkov, D. Pogozhykh, N. Hofmann, T. Mueller, B. Glasmacher. Cryopreservation of stem cells inside alginate 3D constructs after high-voltage encapsulation. Cryobiology 2013;67(3): 429
  • O. Gryshkov, D. Pogozhykh, N. Hofmann, B. Glasmacher. Cell encapsulation into alginate micro-capsules provides living cells with a mild environment during cryopreservation. Cryobiology 2013;66(3): 352
  • H. Zernetsch, A. Repanas, A. Gryshkov, F. Al Halabi, T. Rittinghaus et al. Solving Biocompatibility Layer by Layer: Designing Scaffolds for Tissues. Biomedizinische Technik. Biomedical engineering 2013, doi: 10.1515/bmt-2013-4065
  • O. Gryshkov, N. Hofmann, B. Glasmacher. Electropsraying as a promissing technique for cell encapsulation in semi-permeable micro-capsules for immunoisolation. Cryo Letters 2013;34(2): 186

2012

  • O. Gryshkov, N. Hofmann, B. Glasmacher. Cell encapsulation in semi-permeable alginate micro-capsules using high-voltage processes. Problems of Cryobiology 2012;22(3): 346
  • O. Gryshkov, N. Hofmann, B. Glasmacher. Alginate encapsulation technology improves survival of living cells post-cryopreservation. Problems of Cryobiology 2012;22(3): 345

Presentations (* - presenting author)

2019

  • O. Gryshkov*, V. Mutsenko, S. Suresh, B. Glasmacher. Cryopreservation of cell-seeded electrospun materials: towards biobanking of tissue-engineered constructs. XLVI ESAO 2019 Congress, 05-07.09.2019, Hannover, Germany (05.09.2019, O176, oral).
  • O. Gryshkov*, M. Müller, S. Leal-Marin, V. Mutsenko, S. Suresh, V.M. Kapralova, B. Glasmacher. Advances in the application of electrohydrodynamic fabrication for tissue engineering. International Conference on Emerging Trends in Applied and Computational Physics, ETACP-2019, 21-22.03.2019, Saint-Petersburg, Russian Federation (21.03.2019, invited).

2018

  • O. Gryshkov*. On advancing cooperation with Ukraine. 1st International Scientific and Practical Conference “Information Systems and Technologies in Medicine”, Kharkiv, Ukraine, 28-30.11.2018. (30.11.2018, invited).
  • O. Gryshkov*, M. Tymkovych, O. Avrunin, B. Glasmacher. Image-series analysis for recrystallization process evaluation. 1st International Scientific and Practical Conference “Information Systems and Technologies in Medicine”, Kharkiv, Ukraine, 28-30.11.2018 (29.11.2018, oral).
  • M.V. Prykhodko, M.Y. Tymkovych, O.G. Avrunin, V.V. Mutsenko, O. Gryshkov*, B. Glasmacher. Image processing for automated microscopic analysis of ice recrystallization process during isothermal annealing. 11th International Conference on Bioelectromagnetism (ICBEM), 23-25.05.2018, Aachen, Germany (poster 77).
  • Ya.V. Nosova, O.G. Avrunin*, N.O. Shusliapina, O. Gryshkov, B. Glasmacher. The distribution of the velocity and pressure fields in the nasal cavity at different respiration modes.11th International Conference on Bioelectromagnetism (ICBEM), 23-25.05.2018, Aachen, Germany (poster 76).
  • V.M. Kapralova*, N.L. Vaskova, E.B. Shadrin, A.V. Ilinsky, O. Gryshkov, B. Glasmacher. Cerebrospinal fluid thermoimpedancemetry as a method of brain diseases diagnostics. 11th International Conference on Bioelectromagnetism (ICBEM), 23-25.05.2018, Aachen, Germany (poster 74).
  • I.S. Bondarenko, O.G. Avrunin*, O. Gryshkov, B. Glasmacher. Possibilities of joint application of acoustic radiation and direct magnetic field for biomedical research. 11th International Conference on Bioelectromagnetism (ICBEM), 23-25.05.2018, Aachen, Germany (poster 75).
  • F. Al Halabi, O. Gryshkov, A. I. Kuhn, V. M. Kapralova, B. Glasmacher*. Piezoelectric properties of PVDF and PVDF-TrFE electrospun materials for nerve regeneration. 13th Russian-German Conference on Biomedical Engineering (RGC), 23-25.05.2018, Aachen, Germany (25.05.2018, oral 73).
  • O. Gryshkov*, V. Mutsenko, M. Tymkovych, D. Tarusin, V. Sirotinskaya, I. Braslavsky, O. Avrunin, B. Glasmacher. Advances in cryopreservation of alginate-encapsulated stem cells and analysis of cryopreservation outcome. The 55th Annual Meeting of the Society for Cryobiology, 10-13.07.2018, Madrid, Spain (12.07.2018, oral 254/S135).
  • O. Gryshkov*, M. Tymkovych, O. Avrunin, B. Glasmacher. Optimal cryopreservation yields intact alginate micro-spheres and high cell viability after thawing. The 55th Annual Meeting of the Society for Cryobiology, 10-13.07.2018, Madrid, Spain (poster 256).
  • V. Mutsenko*, A. Barlič, T. Pezić, J. Dermol-Černe, B. Sydykov, O. Gryshkov, W.F. Wolkers, D. Miklavčič, B. Glasmacher. Me2SO- and serum-free cryopreservation of mesenchymal stromal cells using electroporation of sugars. The 55th Annual Meeting of the Society for Cryobiology, 10-13.07.2018, Madrid, Spain (12.07.2018, oral 259/S136).
  • V. Mutsenko*, O. Gryshkov, B. Sydykov, A. Beck, D. Dipresa, A. Lode, T. El Khassawna, A. Petrenko, S. Korossis, W.F Wolkers, M. Gelinsky, B. Glasmacher. ‘In air’ cryopreservation of mesenchymal stromal cells on 3D collagen-hydroxyapatite-scaffolds. The 55th Annual Meeting of the Society for Cryobiology, 10-13.07.2018, Madrid, Spain (12.07.2018, oral 255/S95).
  • V. Mutsenko*, D. Tarusin, B. Sydykov, D. Zaragotas, A. Simaioforidou, C. Rozanski, O. Gryshkov, W.F. Wolkers, I. Braslavsky, E. Anastassopoulos, B. Glasmacher. Study of natural cryoprotective agents for cryopreservation of 3D tissue-engineered scaffolds. The 55th Annual Meeting of the Society for Cryobiology, 10-13.07.2018, Madrid, Spain (poster 263).
  • S. Suresh*, MS. Balamuruganandam, O. Gryshkov, B. Glasmacher. Impact of heterogeneous microstructure of PCL/gelatin electrospun scaffolds on the infiltration of fibroblasts. XLV ESAO Congress Madrid, 12-15.09.2018, Madrid, Spain (oral).
  • C. Esch, M. Müller*, O. Gryshkov, S. Knigge, B. Glasmacher. Visualization Of Hemodynamics: Synthesis Of Artificial Erythrocytes With Encapsulated Piv-Particles Via Microfluidic Systems And Electro-Spraying. XLV ESAO Congress Madrid, 12-15.09.2018, Madrid, Spain (14.09.2018, poster P74).
  • A.I. Kuhn, F. Al Halabi, O. Gryshkov, B. Glasmacher*. Modification of electrospun fibrous polyvinylidene fluoride mats with polyaniline to improve piezoelectric conductivity. XLV ESAO Congress Madrid, 12-15.09.2018, Madrid, Spain (14.09.2018, oral O137).
  • B. Glasmacher*, M. Bode, O. Gryshkov, M. Mueller. An Engineer’S Contribution To Efficient Vascular Replacement Grafts. XLV ESAO Congress Madrid, 12-15.09.2018, Madrid, Spain (15.09.2018, oral O96).
  • V. Mutsenko, D. Tarusin, D. Zaragotas, A. Simaioforidou, C. Rozanski, B. Sydykov, D. Ivnev, O. Gryshkov, E. Anastassopoulos*, B. Glasmacher. Application of infrared video thermography for monitoring of freezing/thawing events within 3D collagen-hydroxyapatite scaffolds. SLTB 2018: Annual Meeting of the Society of Low Temperature Biology, Prague, Czech Republic, 06-07.09.2018 (oral).
  • S. Suresh, M.S. Balamuruganandam, O. Gryshkov, B. Glasmacher*. Improving cell infiltration depth in electrospun scaffolds intended for cardiovascular tissue engineering. IUPESM 2018: World Congress on Medical Physics and Biomedical Engineering, 03-08.06.2018, Prague, Czech Republic (poster 708).
  • O. Gryshkov, N. Klyui, V. Temchenko, V. Kasatkin, H.J. Maier, B. Glasmacher*. Bioactive calcium phosphate coatings on biodegradable magnesium using detonation. IUPESM 2018: World Congress on Medical Physics and Biomedical Engineering, 03-08.06.2018, Prague, Czech Republic (poster 727).
  • B. Glasmacher*, F. AL Halabi, O. Gryshkov, A. Avakian, O. Avrunin. Cross-linked alginate structures for engineering of scaffolds for neural tissue engineering. IUPESM 2018: World Congress on Medical Physics and Biomedical Engineering, 03-08.06.2018, Prague, Czech Republic (poster 803).
  • O. Gryshkov, M. Tymkovych, O. Avrunin, B. Glasmacher*. Cryopreservation of stem cells within intact alginate microspheres. IUPESM 2018: World Congress on Medical Physics and Biomedical Engineering, 03-08.06.2018, Prague, Czech Republic (poster 730).

2017

  • V. Mutsenko*, O. Gryshkov, B. Glasmacher. Cryopreservation of amnion-derived multipotent stem cells within alginate- and collagen-based polymeric matrices. SLTB Meeting 2017, 19-20.09.2017, Cambridge, United Kingdom (oral).
  • B. Glasmacher*, O. Gryshkov. Advances in biomedical engineering: own and cooperation highlights. The German-Ukrainian Forum for Young Researchers, 07-12.02.2017, Kharkiv, Ukraine (invited)
  • B. Kranz*, O. Gryshkov, B. Glasmacher. Cryopreservation of stem cells encapsulated in alginate beads / Kryokonservierung von in Alginat eingekapsel-ten Stammzellen. Annual conference of the German Society of Refrigeration, 22-24 November 2017, Bremen, Germany (vortrag)
  • V. Mutsenko, O. Gryshkov, B. Glasmacher*. Effective solutions for cryostorage of specific tissue types. Annual conference of the German Society of Refrigeration, 22-24 November 2017, Bremen, Germany (oral).
  • F. AL Halabi, O. Gryshkov, M. Müller, V. Kapralova, B. Glasmacher*. Force induced piezoelectric effect of PVDF and PVDF-TRFE scaffolds. The 44th ESAO and 7th IFAO Congress, 6-9 September 2017, Vienna, Austria (poster).
  • S. Suresh, M. Balamuruganandam, O. Gryshkov, B. Glasmacher*. Controlled infiltration of cells into electrospun scaffolds fabricated for application in small diameter vascular grafts. The 44th ESAO and 7th IFAO Congress, 6-9 September 2017, Vienna, Austria (poster).

2016

  • V.V. Mutsenko*, O. Gryshkov, S. Knaack, M. Gelinsky, A.Yu. Petrenko, B. Glasmacher. Cryopreservation of biomimetic bone matrix with non-human primate mesenchymal stromal cells. 53rd Annual Meeting of the Society for Cryobiology – CRYO 2016, 23-27.07.2016, Ottawa, Canada (poster).
  • V.V. Mutsenko*, O.P. Gryshkov, Yu.A. Petrenko, V.P. Grischuk, A. Kotlyarov, P. Schupp, H. Ehrlich, B. Glasmacher, A.Yu. Petrenko. The impact of sucrose pretreatment on survival of mesenchymal stromal cells, cryopreserved in suspension and adherent state. 53rd Annual Meeting of the Society for Cryobiology – CRYO 2016, 23-27.07.2016, Ottawa, Canada (oral).
  • B. Glasmacher*, L. Lauterboeck, D. Saha, A. Chatterjee, S. Suresh, T. Rittinghaus, O. Gryshkov. Cell and tissue encapsulation in alginate for safe cryopreservation. 53rd Annual Meeting of the Society for Cryobiology – CRYO 2016, 23-27.07.2016, Ottawa, Canada (oral).
  • S. Suresh, O. Gryshkov, B. Glasmacher*. Controlled infiltration of cells into spherical polymeric scaffolds. XLIII Congress of the European Society for Artificial Organs, 14-17.09.2016, Warsaw, Poland (poster).
  • V.V. Mutsenko*, O. Gryshkov, S. Knaack, M. Gelinsky, A.Yu. Petrenko, B. Glasmacher. Cryosensitivity of tissue-engineered collagen constructs based on multipotent stromal cells. SLTB Meeting 2016, 07.09.2016, Dresden, Germany (oral).
  • L. Lauterboeck*, O. Gryshkov, N. Hofmann, B. Glasmacher. Importance of controlled ice formation for efficient cell biobanking. ILK 2016, 8-9.09.2016, Dresden, Germany (oral).
  • O. Gryshkov, N. Klyui, V. Temchenko, B. Glasmacher*. Deposition of bioactive and bioinert ceramic coatings on magnesium using detonation. "Dreiländertagung" of Swiss, Austrian and German Societies of Biomedical Engineering – BMT 2016, 4-6.10.2016, Basel, Switzerland (poster).
  • S. Suresh, O. Gryshkov, B. Glasmacher*. Development of Volvox spheres for the co-culture of cardiovascular cells for tissue engineering. "Dreiländertagung" of Swiss, Austrian and German Societies of Biomedical Engineering – BMT 2016, 4-6.10.2016, Basel, Switzerland (poster).

2015

  • O. Gryshkov, N. Hofmann, D. Wirth, T. Mueller, B. Glasmacher. Multistructural alginate encapsulation of drugs, cells and cellular therapeutics. ACHEMA 2015, 19 June 2015, Frankfurt, Germany - Vortrag
  • M. Tymkovych, O. Gryshkov, O. Avrunin, et al. An approach to visualize alginate 3D structures with encapsulated cells for cell-based therapies and cryopreservation. 11th German-Russian Conference on Biomedical Engineering, 17-19 June 2015, Aachen, Germany, p. 13-15 - Poster.
  • O. Gryshkov, N. Klyui, V. Temchenko, et al. Deposition of bioactive coatings on porous bio-SiC ceramics derived from wood. 11th German-Russian Conference on Biomedical Engineering, 17-19 June 2015, Aachen, Germany, p. 107-109 - Poster.

2014

  • O. Gryshkov, D. Pogozhykh, N. Hofmann, T. Mueller, B. Glasmacher. Cryopreservation of monkey mesenchymal stem cells inside alginate 3D microspheres after a high voltage encapsulation. 2014 World Forum on Biology – Joint Meeting of the Society for In Vitro Biology and the Society for Cryobiology, 02.01.2014, Savannah, Georgia - Vortrag (Peter L. Steponkus Crystal Award Presentations).

2013

  •  O. Gryshkov, N. Klyui, V. Temchenko, F. Evertz, V. Dubok, et al. Bioactive and bioinert coatings onto magnesium substrates obtained by gas-detonation deposition: surface morphology studies. 1st Russian-German Conference on Biomedical Engineering, October 23rd – 26th, 2013, Hannover, Germany, p. 5 - Vortrag
  • O. Gryshkov, H. Zernetsch, N. Hofmann, D. Pogozhykh, B. Glasmacher. Engineering of artificial perfusion membrane for biomedical applications. 1st Russian-German Conference on Biomedical Engineering, October 23rd – 26th, 2013, Hannover, Germany, p. 85 - Poster
  • O. Gryshkov, D. Pogozhykh, T. Mueller, N. Hofmann, B. Glasmacher. Encapsulation of mesenchymal stem cells into alginate 3D constructs helps to overcome challenges during cryopreservation. The 3rd International Congress on Controversies in cryopreservation of stem cells, reproductive cells, tissue and organs (Cryo), Berlin, Germany, March 21st-23rd 2013 - Poster

2010

  • N.I. Klyui, V.P. Temchenko, O.P. Gryshkov et al. Characteristics of the hydroxyapatite gas-detonation coatings, deposited onto titanium substrates. Ukrainian-German Symposium on Physics and Chemistry of Nanostructures and on Nanobitechnology. Beregove, The Crimea, Ukraine, 6th-10th September, 2010, p. 214 - Poster.

 Honors, Awards, Fellowships

Press

  •  V. Mutsenko, O. Gryshkov. Cooperation does matter. REBIRTH Newsletter 2018:1; 20.
  • O. Gryshkov. Strengthening cooperation with Ukraine. REBIRTH Newsletter 2017:3; 18-19.
  • O. Gryshkov. F. Evertz, B. Glasmacher. “Mit 1200 m/s ans Ziel: Neuartige bioaktive Beschichtungen für medizinische Anwendungen“ in „Neu Materialien“, Unimagazin,  Leibniz Universitaet Hannover, 1/2, 2014, p. 56-59
  • O. Gryshkov. “Arbeiten in REBIRTH/Working in REBIRTH”.  REBIRTH Newsletter, January 2013, p. 24

Cooperation

Cooperation with Ukraine

External Links

Further activities

  • since 2015 - speaking person of the research group Low temperature applications / Cryotechnology
  • supervisor of Frozen Life project with Saint Ursula School, Hannover