CryoStore: New techniques to visualise and characterise freeze damage using multiphoton microscopy

The Institute of Multiphase Processes is leading two projects as part of the European Union's Horizon Europe programme 'CryoStore'. This project is one of them and is developing a novel multiphoton cryomicroscopy technique for reproductive cells. This innovative method aims to better understand what happens during directional freezing. A motorised, temperature-controlled cryostage will be installed on a multiphoton microscope to capture detailed images of cell membrane changes and the integrity of cell contents during cryopreservation. Software-based image processing and analysis using deep learning and neural networks will be used to classify and validate the results. Validation is supported by LLS Rowiak's expertise in confocal laser scanning and multiphoton microscopy.